Patent US2. 00. 50. Enzymatic template polymerization. RELATED APPLICATION . Nov. 2. 3, 1. 99.
A water-soluble conducting complex of polyaniline/Poly (4-styrenesulfonate) (PANI/SPS) was synthesized using a template-guided enzymatic approach. U. S. 0. 8/9. 99,5. Nov. 6,0. 18,0. 18), which is a continuation- in- part of U. S. 0. 8/9. 15,8. 27, filed Aug. GOVERNMENT SUPPORT . The Government has certain rights in this invention. BACKGROUND OF THE INVENTION . Examples of such uses include light- weight energy storage devices, electrolytic capacitors, anti- static and anti- corrosive coatings for smart windows, and biological sensors. However, the potential applications to which polymers can be put have been limited by their lack of solubility and processability. Analysis and manipulation of polynucleotides is expected to have genetic engineering applications and aid in the diagnosis of genetic disease and in the development and improvement of new drugs. For example, deoxyribonucleotides (DNA) exist in living organisms almost exclusively in a double helix conformation. However, many variations in this conformation has been shown to exist (e. A- , B- , C- and Z- type duplexes). The helical structure of a particular duplex is related to its sequence and its environment. Official Full-Text Publication: The Role of Template in the Enzymatic Synthesis of Conducting Polyaniline on ResearchGate, the professional network for scientists.These variations in conformation are thought to be responsible for the binding of molecular species, such as enzymes or regulatory proteins, to DNA. Therefore, methods of modulating the conformation of DNA are expected to have applications in the area of biosensors, molecular recognition and drug development. SUMMARY OF THE INVENTION . The invention also relates to a method of preparing a polynucleotide/polyaniline complex, wherein the polynucleotide/polyaniline complex is formed by combining a substituted or unsubstituted aniline monomer, a polynucleotide template and a redox enzyme, whereby the aniline monomer aligns along the polynucleotide template to form a complex and polymerizes to form a polyaniline, thereby forming the polynucleotide/polyaniline complex. In a specific embodiment, polyaniline is bound to a polynucleotide double helix as a complex. Oxidation of polyaniline (e. Conversely, reducing the polyaniline will cause a double helix associated with it to become more loosely wound. Therefore, complexation of polyaniline to a polynucleotide double helix provides a method of modulating the conformation of the double helix by changing the oxidation state of the polyaniline. The nanowire includes a polynucleotide template and a conductive polymer bound together as a complex. The method includes connecting at least two electrical elements with a polynucleotide and contacting the polynucleotide with an a redox monomer and a redox enzyme. The monomer aligns along the template to form a complex and polymerizes to form a conductive polymer that is complexed to the polynucleotide that connects the electrical elements. The polynucleotide/conductive polymer complex is electrically conductive and, therefore, forms an electrically conductive connection between the electrical elements. The probe hybridizes with the target polynucleotide which causes at least one electromagnetic property of the conductive polymer to be modified. The target polynucleotide is identified by detecting the modified electromagnetic property. First, the polynucleotide can serve as a template upon wlich the monomers preferentially align themselves to form a complex, such as a charge- transfer complex, thereby limiting parasitic branching and controlling the shape of the polymer. In the case of polyaniline, the mechanism of polymerization is primarily para- directed and results in formation of the electrically active form of polyaniline. This preferential alignment provides improved electrical and optical properties of the final polymer complex. Second, the polynucleotide can serve as a large molecular dopant species which is complexed and essentially locked to the polyaniline chains. Current limitations to the actual use of polyaniline in electronic and optical applications largely has been due to poor dopant stability. Small ionic dopants or chromophores that are currently used are known to diffuse away with time and/or conditions. Locking of a large polyelectrolyte dopant (e. Third, the polynucleotide template can improve water solubility of the final polynucleotide/polyaniline complex for environmentally friendly, facile, and inexpensive processing. For example, changing the oxidation state of polyaniline bound to a DNA duplex changes the linear length of a helical turn and, therefore, could be used to study the binding properties of DNA regulatory proteins. BRIEF DESCRIPTION OF THE DRAWINGS . M aniline to 0. 1. M sulfonated polystyrene (SPS)) formed at various p. H's. 4 shows a plot of absorbance versus (SPS)/aniline ratio to find the optimum dopant- to- monomer ratio. A shows the visible absorption and redox behavior of polyaniline/SPS prepared at p. H 4. 0 with increasing p. H. 5. B shows the visible absorbance and redox behavior of polyanilines/SPS prepared at p. H 4. 0 with decreasing p. H. 6. A shows the visible absorbance and redox behavior of a 5. PDAC) alternating with SPS/polyaniline (prepared at p. H 4. 0) with increasing p. H. 6. B shows the visible absorbance and redox behavior of a 5. SPS/polyaniline (prepared at p. H 4. 0) with decreasing p. H. 7. A shows the visible absorbance of polyphenol without SPS versus phenol monomer. Polyphenol precipitated out of solution as a result of polymerization. B shows the visible absorbance of polyphenol/SPS template versus phenol monomer. The polyphenol did not precipitate out of solution. DNA double helix. UV- Vis spectra of DNA and DNA/polyaniline (Pani) during polymerization. CD spectra of DNA and DNA/polyaniline during polymerization. CD spectra of DNA; a mixture of DNA, aniline monomer and horseradish peroxidase (HRP); DNA and NH4. F; and DNA/polyaniline. A shows the CD spectra of DNA/polyaniline as the p. H is increased from 4 to 1. B shows the CD spectra of DNA/polyaniline as the p. H is decreased from 1. A shows the UV- Vis spectra of DNA/polyaniline as the p. H is increased from 4 to 1. B shows the UV- Vis spectra of DNA/polyaniline as the p. H is decreased from 1. DETAILED DESCRIPTION OF THE INVENTION . It will be understood that the particular embodiments of the invention are shown by way of illustration and not as limitations of the invention. The principal features of this invention can be employed in various embodiments without departing from the scope of the invention. All parts and percentages are by weight unless otherwise specified. This mechanism often results in branched polymeric materials which are intractable and have negligible electrical and optical properties. This invention describes a novel template assisted enzymatic polymerization which results in a new class of polyanilines. In general, the polyanilines formed by the enzymatic template guided polymerization described herein are linked at the para- position (see FIG. A method of the invention includes preparing a polynucleotide/polyaniline complex by combining an aniline monomer, a polynucleotide template and a redox enzyme. The monomer aligns along the template to form a complex and polymerizes to form polyaniline, thereby forming the polynucleotide/polyaniline complex. However, other solvents can be include, for example, dimethyl formamide, dimethyl sulfoxide, methanol, ethanol, dioxane, etc. The p. H of the solvent is adjusted to a p. H in a range of between about 4. Preferably, the p. H is between about 4. Examples of suitable buffers include Tris- HCl buffer, sodium phosphate, sodium citrate, etc. When the template used is a polynucleotide, the preferable buffer is sodium citrate. The concentration of enzyme in the reaction mixture is sufficient to significantly increase the polymerization rate of the monomer in the reaction solution. Typically, the concentration of enzyme in the reaction mixture is in a range of between about one unit/ml and about five units/ml where one unit will form 1. H 6. 0 at 2. 0. Examples of suitable enzymes include peroxidases, laccase, etc. Preferred enzymes are peroxidases. A particularly preferred enzyme is horseradish peroxidase. Such monomers are defined herein as . Examples of suitable redox monomers include substituted or unsubstituted anilines and substituted or unsubstituted phenols. Therefore, the polyaniline or polyphenol formed can be substituted or unsubstituted. The monomer can be a neutral compound, a cation or an anion. Further, the monomer can be, for example, a dye, such as an azo compound, or a ligand. Alternatively, an oligomer can be employed rather than a monomer. Mixtures of different monomers, oligomers, or of monomers and oligomers, can also be employed. In one embodiment, oligomers can form from the monomer prior to association or complexation with a template. The concentration of monomer in the reaction mixture generally is in a range of between about 0. M and about 1. 00 m. M. When aniline is the redox monomer, the template facilitates coupling of the aniline monomers predominately at the para position of the aromatic ring. It is believed that binding of aniline monomers can affect polymerization of adjacent monomers along the polynucleotide template, thereby controlling polymerization, and that the negatively charged polynucleotide backbone forms electrostatic bonds with aniline monomers, thus causing them to align along the backbone of the polynucleotide template. When the redox monomers are polymerized a polynucleotide/conductive polymer complex is formed. When aniline monomers are polymerized in the presence of a polynucleotide template a polynucleotide/polyaniline complex is formed. For example, sulfonated polystyrene, sulfonated polystyrene polyion salts, polypeptides, proteins, biological receptors, zeolites, caged compounds, azopolymers, and vinyl polymers, such as polyvinyl benzoic acid, polystyrene sulfonic acid and polyvinyl phosphonates, poly(vinyl phosphonic acid), etc can be suitable templates. The template can be an anion or cation, such as a polyanion or a polycation.
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Hampton Bay Remotes Hampton Bay Remotes So, why would; Carriage House 52 in. Indoor Iron Ceiling Fan Carriage House 52 in. Indoor; Costa Mesa 56 in. Indoor/Outdoor Weathered Zinc Ceiling Fan Costa Mesa 56 in. Cross- Platform Release Notes for Cisco IOS Release 1. M& T - Release 1. M Caveats . This web- based tool provides you with access to the Cisco bug tracking system, which maintains information about bugs and vulnerabilities in this product and other Cisco hardware and software products. Within the Cisco Bug Search Tool, each bug is given a unique identifier (ID) with a pattern of CSCxx. NNNNN, where x is any letter (a- z) and N is any number (0- 9). The bug IDs are frequently referenced in Cisco documentation, such as Security Advisories, Field Notices and other Cisco support documents. Advertising Programmes Business Solutions +Google About Google Google.com Android ICS 'Encryption Unsuccessful'. Cisco 2801 router rebooting frequently. Context-Free Languages The class of context-free languages generalizes the class of regular languages, i.e., every regular language is a context-free language. From JPEG images or flash videos, to PHP pages, mediaboxAdvanced can display your content in an easy-to-style overlay. Uploaded : Rapidgator : Turbobit : Cloudsix : Datafile : 2Shared : 4Shared : Mega : Uploadable : DFiles : Filefactory : Tusfiles : Mediafire : Uptobox : Uploading. This symptom is observed when using WIC-1B-S/T-V3 or. Cisco has released free software. VFX Flash Transition v1.1B-R7 For dvideoedit.co.cc. Subscribe to: Posts (Atom) Social Profiles. ALL SOFTWARE'S OR AVAILABLE OR FREE AND LINKS, HTML, MAC SOFTWARE'S TORRENT FILES, HOT FILE,RAPID. The regulator of G-protein signalling (RGS)-protein family has important roles in GPCR. The C- or R7-subfamily member RGS9. Technical Assistance Center (TAC) engineers or other Cisco staff can also provide you with the ID for a specific bug. You can save searches that you perform frequently. You can also bookmark the URL for a search and email the URL for those search results. Note If the defect that you have requested cannot be displayed, this may be due to one or more of the following reasons: the defect number does not exist, the defect does not have a customer- visible description yet, or the defect has been marked Cisco Confidential. This document contains the following sections: Using the Cisco Bug Search Tool. The Cisco Bug Search Tool enables you to filter the bugs so that you only see those in which you are interested. In addition to being able to search for a specific bug ID, or for all bugs in a product and release, you can filter the open and/or resolved bugs by one or more of the following criteria: Last modified date. Status, such as fixed (resolved) or open. Severity. Support cases. For more information about how to use the Cisco Bug Search Tool, including how to set email alerts for bugs and to save bugs and searches, see Bug Search Tool Help & FAQ. Note. You must have a Cisco. Cisco Bug Search Tool. If you are redirected to a Log In page, enter your registered Cisco. Log In. To search for a specific bug, enter the bug ID in the Search For field and press Enter. To search for bugs related to a specific software release, do the following: a. In the Product field, choose Series/Model from the drop- down list and then enter the product name in the text field. If you begin to type the product name, the Cisco Bug Search Tool provides you with a drop- down list of the top ten matches. If you do not see this product listed, continue typing to narrow the search results. In the Releases field, enter the release for which you want to see bugs. The Cisco Bug Search Tool displays a preview of the results of your search below your search criteria. You can mouse over bugs to see more content about a specific bug. To see more content about a specific bug, you can do the following: – Mouse over a bug in the preview to display a pop- up with more information about that bug.– Click on the hyperlinked bug headline to open a page with the detailed bug information. To restrict the results of a search, choose from one or more of the following filters: Your search results update when you choose a filter. Resolved Bugs—Cisco IOS Release 1. M1. 2Table 1 Resolved Bugs—Cisco IOS Release 1. M1. 2Identifier. Description. CSCut. 42. 64. 5input queue wedged on a SSLVPN enabled router. CSCuw. 81. 64. 8VG2. Protocol based modem pass- through feature is not working. CSCux. 38. 41. 7IOS and IOS- XE IKEv. Fragmentation Do. SCSCup. 18. 06. 2Memory leak in . This search uses the following search criteria and filters: Field Name. Information. Product. Series/Model. Cisco IOS and NX- OS Software => Cisco IOSRelease. M9. Status. Fixed. Severity. 2 or higher. Resolved Bugs—Cisco IOS Release 1. M8. Cisco IOS Release 1. M8 is a rebuild release for Cisco IOS Release 1. M. The bugs in this section are resolved in Cisco IOS Release 1. M8 but may be open in previous Cisco IOS releases. Symptom: When some port- channels go down at the same time on a router, it can cause EIGRP SIA errors. Conditions: The symptom occurs with full mesh four routers which are connected via port- channels. Additionally, it occurs with over five routers which are connected via a partial mesh port- channel. Workaround: Use the port- channel interface settings below: (config)# interface port- channel < port- channel interface number> (config- if)# bandwidth < bandwidth value> (config- if)# delay < delay value> Further Problem Description: If a test is done with a physical interface, not a port- channel, this issue is not seen. Symptom: A Cisco 5. XM may reload unexpectedly. Conditions: This symptom is intermittent and is seen only when the DSPs available are insufficient to support the number of calls. Workaround: Ensure that sufficient DSPs are available for transcoding. Symptom: Auto mpls- lsp- monitor for pathecho fails. Conditions: This symptom occurs due to an internal scheduling error. Workaround: There is no workaround. Symptom: Cisco 1. Cisco IOS Release 1. M4 could experience the following alignment errors for DSL processes: %ALIGN- 3- TRACE: - Traceback= 0x. B0. EF8z 0x. 60. 7B2. E5. 4z 0x. 60. 07. E4z 0x. 60. 07. 39. C4. 2Cz 0x. 61. 65. FBC1. 0z 0x. FFFFD4. Replacing the HWIC- 1. ADSL- M will not resolve this issue. Conditions: This symptom is observed in Cisco 1. IOS image in Cisco IOS Release 1. M4. Workaround: There is no workaround. Symptom: A Cisco router with flexible netflow configured will crash during the removal of FNF in a timing condition. Conditions: This symptom is triggered by a combination of having simultaneous sessions opened to the router, removal of pre- existing cache entries, and issuing the “show flow monitor < flow. Workaround: Allowing configuration or removal of FNF in a single connection to the router or refraining from running any “show flow monitor < flow. Symptom: A Cisco switch may reload when configured for SNMP. Conditions: This symptom is observed when SNMP is configured. Workaround: There is no workaround. Symptom: Memory “Holding” continues to increases on process “IP SNMP”. This could lead to an out of memory crash. Conditions: This symptom only affects Cisco IOS release 1. SG5, and 1. Symptom: Traffic gets dropped across the tunnel interface when you have the following features enabled: – NAT – VRF– IPSec. Conditions: This symptom is observed when crypto map and VRF are applied under physical interface. Workaround: Disable CEF. Symptom: IOS MGCP gateway may take more than 1. CUCM to the secondary CUCM node. During this time, inbound calls to the MGCP gateway fails with ISDN cause - Recovery on timer expiry. Conditions: This symptom is observed when the secondary CUCM node is restarted before the fail over from the primary CUCM node to the secondary node. The TCP connection between the gateway and secondary CUCM will be in CLOSEWAIT state during the time of problem occurrence. Use show tcp brief to verify the status of TCP connection : Router#show tcp brief TCB Local Address Foreign Address (state) 7. CC7. C 1. 92. 0. 2. CLOSEWAIT Workaround: Restart the MGCP process from the gateway by using no mgcp and mgcp commands. Further Problem Description: When the CCMSUB’s service is down (or machine is powered off), CM service sends a TCP FIN to MGCPGW, however from the debug of MGCPGW, the backhaul link between CCMSUB does not refresh as the TCP layer is stuck at CLOSEWAIT. It is confirmed that the MGCP GW is not notified about this at all, or the MGCP GW does not actively check the status the backup backhaul link. Then CCMSUB’s started/powered on/recovered, however the CCMPUB is down/powered off this time. MGCP application itself will failover immediately, so does the backhaul link. However as the backhaul link’s status was not updated as the TCP layer is still in CLOSEWAIT, the backhaul link is in a false OPEN status and CCM will not be able to leverage this gateway to make outbound calls and all incoming calls are being impacted as well. Symptom: Some EIGRP routes may not be removed from the routing table after a route is lost. The route is seen as “active” in the EIGRP topology table, and the active timer is “never”. Conditions: This symptom is seen when a multiple route goes down at the same time, and query arrives from neighbor router. Finally, neighbor detects SIA for affected router and neighbor state is flap. However, active entry is remaining after that, and route is not updated. Workaround: The clear ip eigrp topology network mask command may remove unexpected active entry. Symptom: %SYS- 3- INVMEMINT: Invalid memory action (malloc) at interrupt level. Conditions: The symptom is observed when music on hold (MOH) is enabled. Workaround: Remove the route list from the multicast MOH CLI, so that you can still have music on hold and can continue the feature. Workaround 2: Disabling the MOH (but no music comes on hold). Symptom: When attempting a T. FLEXDSPRM- 3- UNSUPPORTED. Conditions: The symptom is observed with a T. Workaround: There is no workaround. Symptom: During regular operations, a Cisco router running Cisco IOS release 1. T and possibly other releases experiences a crash. The crash info will report the following: %SYS- 2- FREEFREE: Attempted to free unassigned memory at 4. A0. 01. C2. C, alloc 4. C, dealloc 4. 17. B0,%SYS- 6- BLKINFO: Attempt to free a block that is in use blk 4. A0. 01. BFC, words 1. C, Free, dealloc 4. B0, rfcnt 0, Conditions: This symptom is not observed under any specific conditions. Workaround: There is no workaround. Symptom: A memory leak is seen at ssf. The “mpls mtu override” option does not work on the Cisco c. Packets are dropped with the “%LINK- 4- TOOBIG: ” error. The packet size printed in the “%LINK- 4- TOOBIG: ” error is wrong; it is printing wrong parameter instead of the datagram size. It has been seen while updating various different commands such as, those under– call- manager- fallback– ip route statements– interface sub- commands. Workaround: There is no workaround. Symptom: After an upgrade from Cisco IOS Release 1. M to Cisco IOS Release 1. M, the CPU usage with the same traffic load is increased. Conditions: This symptom is observed with the Cisco ISR- G2 platform. |
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